Aim to obtain high titer fermentable sugars from corn stover for future high titer succinic acid production, the effect of steam explosion and dilute acid pretreatment methods on enzymatic hydrolysis were compared in this study at first.Many inhibitors were generated in the pretreatment process and could inhibit the fermentation of succinic acid.Water washing and resin (D301) adsorption methods were used to remove the inhibitors of corn stover hydrolysate.The results indicated that the total sugar concentration in steam explosion pretreated corn stover hydrolysate finally reached 147.24 g/L, which was higher than that of 115.61 g/L in dilute acid pretreated corn stover hydrolysate.25.32 g/L of succinic acid with 42.19% of yield was obtained from detoxified corn stover by only water washing after steam explosion pretreatment，it was 11.59% higher than dilute acid pretreatment group.This study provided the primary succinic acid production from lignocellulosic feedstock with potential of industrial applications, and suggested that the lignocellulose could be an important alternative feedstock used for future industrial succinic acid production.