The enzymatic hydrolysis from Tilapia meat treated by trypsin, mineral ion binding activities and the antioxidant activities were investigated.The results showed that the hydrolyzates (DH=7.41) had the highest mineral ion binding activity.Its Ca2+ combination rate was 78.71%, Fe2+ combination rate was 73.84%, and Zn2+ combination rate was 71.09%.The combinations exhibited some antioxidant capacity.The Ca2+ combinations scavenging rate of DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and hydroxyl radical, as well as reducing power were 77.35%,32.51%,0.29, respectively, which were significantly higher than those of Fe2+, Zn2+ combinations.In addition, the amino acid sequence analysis showed that the hydrolyzates with higher contents of Glu,Asp,Lys and Ser had the higher mineral ion binding activity.