To establish a method for determination ethoxyquin and ethoxyquin dimer in pork by HPLC-FLD.After homogenized, pork was extracted by n-hexane at ratio of 5 ∶15(g∶mL) with ultrasound assistant at room temperature.The extract was dried by vacuum and fixed with acetonitrile.The content of ethoxyquin and thoxyquindimer in the sample was detected by high performance liquid chromatography with C18 chromatographic column; the column temperature was 30 ℃.Fluorescence detector was used and the excitation wavelength was 360 nm, absorption wavelength was 440 nm.Mobile phase of acetonitrile was 2% acetic acid (85∶15,V/V) at 1.0 mL/min flow rate.Under the above conditions, the retention time of ethoxyquin was 5.816 min, and the linear regression equation was: Y=604.27X-8 446, R2=0.993 6.The retention time of ethoxyquin dimer was 32.436 min, and the linear regression equation was Y=1 425.3X-6 140.8, R2=0.995 6.The recovery rate of ethoxyquin was between 93.88%-107.18%, and the recovery rate of ethoxyquin dimer was between 80.74%-83.62%.The RSD is less than 5.00%.This method is simple and rapid, high sensitivity.It can be used for detecting ethoxyquin and ethoxyquin dimer in pork.