To improve the production of lycopene biosynthesis in Saccharomyces cerevisiae, the adaptation between lycopene biosynthesis pathway and normal metabolism of the chassis was optimized through investigating the species of chassis host, the copy number of functional gene and the type of promoter. The results proved that lycopene could be efficiently produced when using Saccharomyces CEN.PK2-1C as chassis, coupling with multi-copy plasmid pRS42K, and employing constitutive promoter to control the expression of lycopene biosynthesis pathway. Comparing with the initial engineered strain, yield of lycopene was increased by about 70 times, reaching 3.62 mg/L, suggesting that improving the adaptation between exogenous metabolic pathway and yeast host was an effective approach to enhance the synthesis of target products.