In order to study the effect of ethanol fumigation on the quality of postharvest Agaricus bisporus, Agaricus bisporus was treated by 200, 400 and 600 μL/L of ethanol during the fumigation. The results showed that ethanol fumigation could significantly inhibit the increase of respiration intensity, reduce consumption of the substrate, delay the increase of weight loss and total number of colonies, effectively alleviate the decrease of ascorbic acid (Vc) content, and maintain stable soluble protein content. At the same time, it can effectively reduce the total phenol content, maintain bisporus whiteness, hardness and sensory score. The 400 μL/L ethanol fumigation treatment showed the best preservation effect．