A method for the determination of 42 kinds of organophosphorus pesticide in plant-based peptide powder by solid-phase extraction-gas chromatography-flame photometric detector(SPE-GC-FPD) was established. The samples were extracted by acetonitrile, concentrated and purified by solid phase extracted on PestiCarb/NH2 column, eluted with acetonitrile/toluene(volume fraction is 75%). Finally, the pesticides were determined by flame photometric detector(FPD), quantified by external standard method. The recoveries were 72.8%-103.8% at 2 spiked levels of 0.05 and 0.50 mg/kg for 42 organophosphorus pesticides, and the relative standard deviations (RSDs) were between 2.44%-8.71%. The limits of detections were in the range of 0.005-0.020 mg/kg and the limits of quantitation were in the range of 0.015-0.060 mg/kg. A total of 10 plant-based peptides was tested and none of these pesticides were detected . The method can be applied to determine organophosphorus pesticides in plant-based peptide powder samples due to its efficiency and simplicity.