An actinomyce, which could degrade β-cypermethrin, was isolated from kale yard soil sprayed pyrethroid pesticides for a long time. According to physic-biochemical characteristics and 16S rDNA sequence, the strain was classified as Streptomyces sp. K-1. Strain K-1 and Bacillus licheniformis B-1 with high degradation capacity on β-cypermethrin were co-cultured, and the appropriate degradation conditions of β-cypermethrin in the system were selected. Suitable conditions were as follows: mixed medium ratio (GSM∶LB, v/v) of 1∶1, inoculation ratio (K-1∶B-1, v/v) of 2∶1, inoculating strain B-1 after inoculation of strain K-1 for 24 hours, and 35 ℃ on a rotary shaker at 150 r/min. Under these conditions, 88.3% β-Cypermethrin (50 μg/mL) could be removed within 72 hours.