In this paper, two lactic acid bacteria, Pediococcus acidilactici CCFM 28 and Lactobacillus gasseri JCM 11657, which show antimicrobial activity independent of pH value were obtained through agar well diffusion test. With treatment of catalase and protease, the neutral supernatant of these two strains still exhibit strong antimicrobial activities. Thus, the antimicrobial substances in the supernatant were mainly bacteriocins. Besides, the inhibition spectrum of CCFM 28 was larger than that of JCM 11657. Furthermore, another two lactic acid bacteria without bacteriacin, P. acidilactici ZT-45 and L. gasseri JS-SZ-1-5, with similar biological properties with CCFM 28 and JCM 11657 such as generation time, ability of resistance to acid and bile salts, were screened as negative control strains for the bacteriocin-producing strains to treat 5-week-old C57BL/6 male mice together with CCFM 28 and JCM 11657. Results from animal experiment suggested that producing bacteriocin or not, the type of bacteriocin or the range of inhibition spectrum all might affect the composition of gut microbiota, but not affect the levels of short chain fatty acids in gut.
YU Yun-xia et al
. Effects of bacteriocin produced by lactic acid bacteria on composition of gut microbiota and metabolites of gut flora in mice[J]. Food and Fermentation Industries, 2018
, 44(7)
: 20
-28
.
DOI: 10.13995/j.cnki.11-1802/ts.016906