The moisture state and content of Tricholoma matsutake after freeze-thaw were analyzed by low-field nuclear magnetic resonance (LF-NMR) technology. The effects of freeze-thaw cycles on the thawing loss rate, color and hardness were compared. To learn the effect of the freeze-thaw process on Tricholoma matsutake moisture distribution, during the experiment period, the proton density weighted images of the sample were obtained with multi-slice spin echo (MSE) pulse sequence. The results showed that the water states in Tricholoma matsutake included free water, immobilized water and bound water, which were T21 (0.01-10 ms), T22 (10-100 ms), T23 (100-1 000 ms), respectively. With the increasing of freeze-thaw cycles, the free water content and immobilized water content was decreased gradually, bound water content was increase slightly and then decreased; The thawing loss rate increased, L values (lightness), a*(redness) and b*(yellowness) values decreased, hardness decreased first and then increased, causing a decline in quality. MRI results showed that water loss of stipe was more serious than that of pileus, some areas of stipe hardly existed free water after 5 cycles of freeze-thaw. The correlation analysis showed that the times of freeze-thaw had a significant correlation (p<0.01) with T22, T23, a*(redness), b*(yellowness) and thawing loss rates. The T22 and T22 had a significant correlation (p<0.05) with thawing loss rates, a*(redness) and b*(yellowness). Therefore, application of using LF-NMR in the determination of water content and composition of Tricholoma matsutake could be used as reference indexes for the quality control.