We extracted and purified the anti-oxidized peptide of Nuodeng ham through Sephadex G-25 gel chromatography. The anti-oxidative activity of components was studied in vitro, including ·OH radical scavenging assay, DPPH radical scavenging assay and chelation Combined Fe2+ experiment. The components with the highest antioxidant activity were separated and purified by Sephadex G-15 gel chromatography. Finally, the antioxidant peptides were identified by MALDI-TOF-MS. Results: Noudeng ham antioxidant peptide were separated and purified by Sephadex G-25 gel chromatography to obtain four components, namely A, B, C and D. Component C had the strongest antioxidant activity. The free radical scavenging rate, DPPH radical scavenging rate and Fe2+ chelation rate reached 50.01%, 48.32% and 34.37% when the concentration of component C was 1 mg/mL, respectively. Compared with other components (A, B and D), the difference was significant (p<0.01). The component C was Purified by Sephadex G-15 gel chromatography of the five components (C1, C2, C4 and C5) , wherein the component C3 has the highest antioxidant activity. The free radical scavenging rate, DPPH radical scavenging rate and Fe2+ chelation rate reached 73.01%, 51.21% and 65.23% when the concentration of component C3 was 1 mg/mL, respectively. Compared with other components (C1, C2, C4 and C5), the difference was significant (p<0.01). The antioxidant peptides of C3 were identified by MALDI-TOF-MS.