Isolation, purification and identification of antioxidant peptide from Nuodeng ham

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  • 1( College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China)2( Livestock Product Processing Engineering and Technology Research Center of Yunnan Provine, Yunnan Agricultural University, Kunming 650201, China)

Online published: 2018-09-05

Abstract

We extracted and purified the anti-oxidized peptide of Nuodeng ham through Sephadex G-25 gel chromatography. The anti-oxidative activity of components was studied in vitro, including ·OH radical scavenging assay, DPPH radical scavenging assay and chelation Combined Fe2+ experiment. The components with the highest antioxidant activity were separated and purified by Sephadex G-15 gel chromatography. Finally, the antioxidant peptides were identified by MALDI-TOF-MS. Results: Noudeng ham antioxidant peptide were separated and purified by Sephadex G-25 gel chromatography to obtain four components, namely A, B, C and D. Component C had the strongest antioxidant activity. The free radical scavenging rate, DPPH radical scavenging rate and Fe2+ chelation rate reached 50.01%, 48.32% and 34.37% when the concentration of component C was 1 mg/mL, respectively. Compared with other components (A, B and D), the difference was significant (p<0.01). The component C was Purified by Sephadex G-15 gel chromatography of the five components (C1, C2, C4 and C5) , wherein the component C3 has the highest antioxidant activity. The free radical scavenging rate, DPPH radical scavenging rate and Fe2+ chelation rate reached 73.01%, 51.21% and 65.23% when the concentration of component C3 was 1 mg/mL, respectively. Compared with other components (C1, C2, C4 and C5), the difference was significant (p<0.01). The antioxidant peptides of C3 were identified by MALDI-TOF-MS.

Cite this article

WU Bao-sen et al . Isolation, purification and identification of antioxidant peptide from Nuodeng ham[J]. Food and Fermentation Industries, 2018 , 44(7) : 102 -109 . DOI: 10.13995/j.cnki.11-1802/ts.015594

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