The situation of Lentinus edodes′ hyphae, growth cycle, conversion percent and contamination rate were used as indices to determine the formulation for cultivation of Lentinus edodes with distiller′s grain as the main raw materials. The quality of Lentinus edodes was evaluated on the basis of the conventional nutrients, amino acid content and volatile components. The security of this method was also assessed based on the content of heavy metals and pesticide residues. And the results showed that cultivation of Lentinus edodes using distiller′s grain could result in' high yield, high quality and as well as safety.