Maize bran is one of the important feedstuff, because of it contains lots of non-starch polysaccharides, resulting in the decrease of feed digestion and absorption rate and it seriously restrict its application in feed industry. The mechanical pretreatment of maize bran can improve its feed utilization rate and promote the subsequent biological utilization, which has important economic benefits. Effects of physical properties, chemical properties, and enzymatic efficiency by ball milling and blade crushing from maize bran was investigated in this paper. The results showed that the maize bran were crushed by ball milling and blade crushing, the total soluble sugar contents of maize bran were respectively increased 1.6 times and 2.0 times, reducing sugar contents were respectively increased by 1.8 times and 2.6 times, and the contents of feruloyl oligosaccharides was increased by 1.1 times and 1.6 times, respectively. With xylanase and cellulase hydrolysis of maize bran after ball milling and blade crushing, the total soluble sugar contents were increased by 2.2 fold and 3.0 fold, reducing sugar contents were increased by 2.5 fold and 3.7 fold, and feruloyl polysaccharide contents were increased by 1.3 fold and 2.0 fold, respectively, compared to those of maize bran after enzymatic hydrolysis without mechanical treatment. The efficiency of enzymatic hydrolysis of maize bran after blade crushing was higher than that of maize bran after ball milling.