An ultra-performance liquid chromatography (UPLC) method was developed for simultaneous detection of 9 kinds of sulfonamides and 3 kinds of fluoroquinolones in pork. Ground pork was extracted in phosphate solution (pH=6), purified by HLB solid phase extraction column; after nitrogen drying at 40 ℃, 20% methanol-water solution was used to dissolve to 1 mL; 0.1% formic acid aqueous solution and acetonitrile were used as the mobile phasesolution, UV tandem fluorescence detectors was used as a detector. Sulfonamides and fluoroquinolones showed good linear in the range of 0.01-1.00 μg/mL, and 0.001-0.200 μg/mL, respectively, with the correlation coefficients above 0.999. The limits of detection (LOD) of sulfonamides were 1.2-3.5 μg/kg and the limits of quantitation (LOQ) of sulfonamides were 4.0-11.7 μg/kg, the limits of detection (LOD) of fluoroquinolones were 0.12-0.50 μg/kg and the limits of quantitation (LOQ) of fluoroquinolones were 0.4-1.7 μg/kg, the recoveries of spiked samples were between 70.0%-98.0% and the relative standard deviations (RSD) was less than 5% (n=6). The UPLC method established in this experiment can detect 12 kinds of targets simultaneously. This method exhibited good symmetrical peak shape, low detection limit, good reproducibility, high accuracy, short analysis time and fewer solvents. The method can be widely used for simultaneous detection of sulfonamides and fluoroquinolones in meat and its products.