In this paper, a real-time PCR method based on SYBR Green I has been successfully established for the identification of bovines milk components in goat dairy products. Two pairs of specific primers targeted at mitochondrial 12S rRNA genes were selected and DNA extracts from fresh bovine and goat milk samples were used as PCR templates. The results showed that the proposed method can detect the adulteration level of bovine milk as low as 2.5% in fresh goat milk. The method was then applied in the identification of ingredients derived from bovine and goat in 11 commercial dairy products. This SYBR Green I-based real-time PCR method does not need the complicated electrophoresis separation and expensive labeled-DNA probes. It has the advantages of rapid, sensitive, low cost and high throughput. The method provides an effective technology for the adulteration in the goat milk industry.