Food and Fermentation Industries >

2018 , Vol. 44 >Issue 8: 1 - 7

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.016910

The effect of different expression patterns on B-type natriuretic peptide expression in Corynebacterium glutamicum

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  • 1(School of Biotechnology, Jiangnan University, Wuxi 214122, China)2(National Engineering Laboratory of Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China)3(Key Laboratory of Industrial Biotechnology, Jiangnan University, Wuxi 214122, China)

Online published: 2018-09-13

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Abstract

For researching the expression model of B-type natriuretic peptide (BNP) in Corynebacterium glutamicum, a fast protein expression system was constructed using GFP as a model protein. The results showed that constitutive intracellular patterns were the best way for BNP expression compared with inducible intracellular, constitutive secretory and inducible secretory patterns. The BNP was purified and its production was about 235.5 mg/L with high purity (>90%). Conclusively, a fast protein expression system with different expression patterns was constructed in C. glutamicum, and BNP was successfully expressed using this system. This will greatly facilitate the application of this important platform organism.

Key words： Corynebacterium glutamicum; BNP; protein expression; expression patterns

Cite this article

PENG Feng et al . The effect of different expression patterns on B-type natriuretic peptide expression in Corynebacterium glutamicum[J]. Food and Fermentation Industries, 2018 , 44(8) : 1 -7 . DOI: 10.13995/j.cnki.11-1802/ts.016910

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