Fruiting body components of Agrocybe cylindracea between the first three tides were analyzed and compared by the national standard(GB) test method according to FAO and WHO evaluation criteria. The results showed that crude protein contents in the fruiting body of first tide were higher than those in the second and third tides, but the crude fat content were not significant difference among three tides. Amino acid composition of the fruiting body of three tides were identical in terms of category but different in the content. The contents of total amino acids of the third tide were significantly higher than that in the other two tides. Heavy metals (Pb, Cd, Hg and As) contents in fruiting body of the third tide were significantly higher than the other tides, but the contents in all of the three tides did not exceed national food safety standards, and met the requirements of green food (edible mushrooms). By evaluation of these indicators, we know that quality of the third tide of Agrocybe cylindracea was better on protein and amino acids concentrations, but the heavy metals contents was also higher.