The aim of this study was to screen lactic acid bacteria (LAB) inhibiting Fusarium graminearum ACCC36938 and a strain LAB A14-2 with strong antifungal effect was obtained. The effects of different temperature, pH value and protease treatment on the antifungal activity of LAB A14-2 were further studied. The results showed that the pH value had the greatest effect on the antibacterial activity of LAB A14-2, and non-protein heat-sensitive substances also had some antifungal activity. In order to investigate the effect of LAB A14-2 on the biosynthesis of deoxynivalenol (DON), LAB A14-2 and Fusarium graminearum were co-cultured on wort medium and DON content changes were analyzed. The results showed that LAB culture and supernatant were able to inhibit the growth of Fusarium graminearum and significantly reduce the amount of DON synthesis, but LAB cell can't bind DON. Finally, physiological and biochemical characteristics and molecular identification of LAB A14-2 showed that it was Lactobacillus plantarum.
LUO Wei
,
SONG Chun-yan
,
LI Yan-lin
,
ZHANG Wei
,
LU Xin-yi
,
CAO Yu
. Screening and identification of lactic acid bacteria inhibiting the biosynthesis of deoxynivalenol[J]. Food and Fermentation Industries, 2018
, 44(9)
: 41
-47
.
DOI: 10.13995/j.cnki.11-1802/ts.017076
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