Three lactic acid bacteria with strong antibacterial activity were selected from traditional fermented foods. The activity of bacteriocin produced by the strain identified and named as Lactobacillus plantarum WZS02 was only reduced by about 8% after being treated at 121 ℃ for 30 min. Ionic exchange and preparative liquid chromatography were used to separate and purify the bacteriocin produced by this strain. The titer and antibacterial spectrum of bacteriocin WZS02 were determined. When 1 mg/mL Nisin standard (1 000 IU/mg) was used as a reference, the titer of the fermentation broth of strain WZS02 was 9 097 IU/mL with Escherichia coli as the indicator, and 2 796 IU/mL with Staphylococcus aureus as the indicator. It was found that the bacteriocin WZS02 had strong resistance to Gram-negative and positive bacteria. The bacteriocin has a strong application potential.
SUN Jie
,
JIANG Jie
,
FEN Bin-bin
,
KONG Ling-Min
,
WANG Zhao
,
WEI Chun
. Screening of a bacteriocin-producing lactic acid bacteria and characteristics of the bacteriocin[J]. Food and Fermentation Industries, 2018
, 44(9)
: 48
-52
.
DOI: 10.13995/j.cnki.11-1802/ts.017321
[1] 李平兰, 张篪, 江汉湖. 乳酸菌细菌素研究进展[J]. 微生物学通报, 1998, 25(5):295-298.
[2] 吕利军, 季海峰, 杨英, 等. 乳酸菌微胶囊及其合生素对仔猪生产性能和免疫力的影响[J]. 畜牧与兽医, 2009, 41(2):35 - 37.
[3] 张晓巩, 方超, 白卉, 等. 抗菌肽作用机制的研究进展[J]. 生理科学进展, 2011, 42(1):11-15.
[4] BOLINTINEANU D, HAZRATI E, DAVIS H T, et al. Antimicrobial mechanism of pore-forming protegrin peptides: 100 pores to kill E. coli[J]. Peptides, 2010, 31(1):1-8.
[5] UYTERHOEVEN E T, BUTLER C H, KO D, et al. Investigating the nucleic acid interactions and antimicrobial mechanism of buforin II[J]. Febs Letters, 2008, 582(12):1 715-1 718.
[6] ANBANANDAM A, ALBARADO D C, TIRZIU D C, et al. Molecular basis for proline- and arginine-rich peptide inhibition of proteasome[J]. Journal of Molecular Biology, 2008, 384(1):219-227.
[7] GUEGUEN Y, BERNARD R, JULIE F, et al. Oyster hemocytes express a proline-rich peptide displaying synergistic antimicrobial activity with a defensin[J]. Molecular Immunology, 2009, 46(4):516-522.
[8] CUTHBERTSON B J, DETERDING L J, WILLIAMS J G, et al. Diversity in penaeidin antimicrobial peptide form and function[J]. Developmental & Comparative Immunology, 2008, 32(3):167-181.
[9] BRÖTZ H, BIERBAUM G, MARKUS A, et al. Mode of action of the lantibiotic mersacidin: inhibition of peptidoglycan biosynthesis via a novel mechanism[J]. Antimicrobial Agents & Chemotherapy, 1995, 39(3):714-719.
[10] 凌代文, 东秀珠. 乳酸细菌分类鉴定及实验方法[M]. 北京:中国轻工业出版社, 1999.
[11] VAN REENEN C A, DICKS L M T, CHIKINDAS M L. Isolation, purification and partial characterization of plantaricin 423, a bacteriocin produced by Lactobacillus plantarum[J]. Journal of Applied Microbiology, 1998, 84(6):1 131.
