In this paper, microcapsules of two Lactobacillus strains producing acetaldehyde dehydrogenase (ALDH), Lactobacillus plantarum and Lactobacillus acidophilus, were prepared using calcium chloride extrusion technique with sodium alginate and skimmed milk powder as encapsulants. The protective effect of microcapsules on acetaldehyde dehydrogenase in artificial gastric and intestinal succus in vitro was examined. The results showed that the average particle size of double-Lactobacillus alginate microcapsules was 1.14 mm, the appearance of which was round and full, and the embedding rate reached 99.8%. The ALDH activity from Lactobacillus microcapsules maintained 95% and 73.4% in the artificial gastric succus system after 90 min and 180 min, respectively. However, the ALDH activity of free bacteria in the artificial gastric succus system decreased to zero after 30 min. In the artificial intestinal succus, caalginate embedded Lactobacillus microcapsules had favorable enteric solubility. The retention rate of the ALDH activity was still 97.8% and 86.5% after digestion for 30 min and 50 min.
WAN Yin
,
LUO Cheng
,
ZHANG Hong-ting
,
GUO Shuai-ling
,
PI Xiao-wen
,
TONG Huo-yan
,
FU Gui-ming
. Protection of Lactobacillus ALDH activity by sodium alginate microcapsule during gastrointestinal digestion in vitro[J]. Food and Fermentation Industries, 2018
, 44(9)
: 61
-65
.
DOI: 10.13995/j.cnki.11-1802/ts.015646
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