Echinocandin B (ECB) is the key precursor of anidufungin, mainly synthesized by microbial fermentation. In this experiment, the scale-up conditions of Aspergillus nidulans ZJB09223 for ECB fermentation was optimized in the fermentor. The effects of different types of agitating paddles, stirring speed and aeration volume on the Echinocandin B fermentation were investigated to achieve the basic conditions of industrialization. The comparison of different impeller types showed that flat-straight leaf disc tubine type agitator was more suitable for ECB fermentation process. The optimum aeration amount was 1.7 VVM, and the stirring speed was 600 r/min in the first 6 days and 400 r/min in the next 9 days to obtain the best results of 608 mg/L, which was 193% higher than that under the original conditions.
HU Xiao-long
,
QIN Hai-bin
,
WU Xu-ping
,
MAO Jian
,
ZOU Shu-ping
,
NIU Kun
. Optimization of the fermentation parameters for Echinocandin B production in 5 L bioreactor[J]. Food and Fermentation Industries, 2018
, 44(9)
: 130
-135
.
DOI: 10.13995/j.cnki.11-1802/ts.017467
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