Strategies for feeding control in microbial production of scleroglucan bySclerotium rolfsii WSH-G01

  • GAO Rong-wei ,
  • ZHOU Jing-wen ,
  • XU Guo-qiang.
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  • 1 (Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China) 2 (National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China)

Received date: 2018-03-11

  Revised date: 2018-04-08

  Online published: 2018-11-23

Abstract

In order to improve the production of scleroglucan by Sclerotium rolfsii WSH-G01 using glucose as the substrate, on the basis of the optimal stirring speed, the effects of two feeding methods on biomass and titer of polysaccharides were studied. The stirring speed was 400 r/min. The best feeding strategy was maintaining the feeding speed of 6 g/(L·h) for 32 h after 40 h of fermentation when the total glucose concentration was 400 g/L. The results showed that the highest titer of polysaccharide (32.62 g/L), the biomass (22.36 g/L), the productivity (0.45 g/(L·h)), and the substrate utilization rate (28%) were reached at 72 h. The polysaccharide production was 15% higher than that obtained using the other feeding method and increased by 76.5% compared with batch fermentation.

Cite this article

GAO Rong-wei , ZHOU Jing-wen , XU Guo-qiang. . Strategies for feeding control in microbial production of scleroglucan bySclerotium rolfsii WSH-G01[J]. Food and Fermentation Industries, 2018 , 44(10) : 51 -56 . DOI: 10.13995/j.cnki.11-1802/ts.017234

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