In order to improve the production of scleroglucan by Sclerotium rolfsii WSH-G01 using glucose as the substrate, on the basis of the optimal stirring speed, the effects of two feeding methods on biomass and titer of polysaccharides were studied. The stirring speed was 400 r/min. The best feeding strategy was maintaining the feeding speed of 6 g/(L·h) for 32 h after 40 h of fermentation when the total glucose concentration was 400 g/L. The results showed that the highest titer of polysaccharide (32.62 g/L), the biomass (22.36 g/L), the productivity (0.45 g/(L·h)), and the substrate utilization rate (28%) were reached at 72 h. The polysaccharide production was 15% higher than that obtained using the other feeding method and increased by 76.5% compared with batch fermentation.