Chemical synthetic preservatives and natural preservatives are the two main preservatives in China′s food industry. With the concern of food safety problems caused by chemical synthetic preservatives, natural preservatives became a research hot spot because of their environmental protection, safe and non-toxicity. Oregano essential oil as a natural plant preservative rapidly developed for its broad-spectrum antibacterial effect and antioxidant activity in food preservation industry. Based on the characteristics of oregano essential oil and their preservation mechanism, this paper discusses its application in preservation of fruits and vegetables, meat products, eggs and cheese. The problems of oregano essential oil in food preservation was also discussed, it provides a theoretical basis for the further use of oregano essential oil.