In order to elucidate the effects of the genes encoding key enzymes in the porphyrin synthetic pathway as well as environmental factors on the regulation of porphyrin metabolism in E.coli, genes of gltX, hemA, hemB, hemD hemH and hemA-hemD were homogenously overexpressed respectively, and the effects of environmental factors on the precursor 5-aminolevulinic (ALA) and heme biosynthesis were analyzed. The results showed that the overexpression of hemA promoted the synthesis of heme obviously, and the effects of temperature and dissolved oxygen on the production of ALA and heme in Eco/pEA, a hemA overexpressed strain, were inconsistent. Under the optimal condition, the heme content in Eco/pEA was 11.7 fold of that in the parent strain. Therefore, the differences were significant among gene-overexpressing strains under different environmental conditions in porphyrin metabolism.
CHEN Dan-yuan
,
SHEN Yun-jie
,
YANG Yan
,
TANG Lei
. Regulation of heme synthesis in Escherichia coli by overexpression of genes for the key enzymes and environmental factors[J]. Food and Fermentation Industries, 2018
, 44(11)
: 7
-14
.
DOI: 10.13995/j.cnki.11-1802/ts.017222
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