Lactobacillus reuteri is one of the most important probiotics in human body. The nitrogen source is the key factor restricting its fermentation production. The study of types and characteristics of nitrogen sources effectively used by Lactobacillus reuteri will further guide the fermentation. The utilization efficiency of Lactobacillus reuteri 138-1 for 21 kinds of marketable nitrogen sources was analyzed by batch culture system. The changes of amino acids and peptides of each nitrogen source before and after used by Lactobacillus reuteri 138-1 during constant pH and automatic feedback feeding culture were determined by high performance liquid chromatography (HPLC). The types of available nitrogen source and the molecular weight of peptides utilized by the strain were analyzed. Lactobacillus reuteri 138-1 mainly used small peptides with molecular weight below 1 000 Da, and had the highest utilization rate for the peptides below 500 Da. Although all nitrogen sources were rich in small molecular peptides, the nitrogen utilization of the strain had the substrate specificity. The small molecular peptides in yeast peptone such as yeast extract, yeast protein 103, yeast extract 528 and yeast extract 803 were more effectively utilized. The utilization efficiency of yeast nitrogen source was significantly higher than that from plant, dairy and animal tissues.
ZHU Dan-feng
,
WANG Yuan-yuan
,
CUI Shu-mao
,
MAO Bin-yong
,
ZHAO Jian-xin
,
ZHANG Hao
,
CHEN Wei
. Selectivity and characteristic analysis of nitrogen source utilized by Lactobacillus reuteri[J]. Food and Fermentation Industries, 2018
, 44(11)
: 35
-41
.
DOI: 10.13995/j.cnki.11-1802/ts.017734
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