The differential expression of genes associated with fatty acids metabolism in Thermoactinomyces daqus at different temperatures were analyzed by RNA-seq technology combined with quantitative real-time PCR, and the composition changes of the fatty acids in Thermoactinomyces daqus at different temperatures were determined by the Gas chromatography combined with Sherolock automatic bacterial identification system. The results showed the key genes involved in fatty acid synthesis were up-regulated by 7.3 times at 55 ℃ and up-regulated 8.4 times at 60℃ while the key genes involved in the β-oxidative decomposition of fatty acids were down-regulated by 7.3 times at 55 ℃ and 7.6 times at 60 ℃ compared with 45 ℃. The key genes involved in decomposition of branched chain amino acids were down-regulated by 5.3 times at 55 ℃ and 6.0 times at 60℃ while the key genes involved in the Acetyl-CoA synthesis were up-regulated by 2.7 times at 55 ℃ and 3.1 times at 60 ℃ compared with 45 ℃. The Thermoactinomyces daqus mainly contained straight chain saturated fatty acid and monomethyl branched saturated fatty acid. With the increase of temperature, the branched-chain saturated fatty acids in Thermoactinomyces daqus showed a downward trend and the Straight chain saturated fatty acids showed an upward trend. The contents of C16:0 and C18:0 in Thermoactinomyces daqus were respectively increased by 1.6 and 10 times at 60 ℃ compared with 45 ℃.
FENG Hui-jun
,
ZHAI Lei
,
YU Xue-jian
,
CHENG Kun
,
LIU Yang
,
YAO Su
. Fatty acids metabolism changes of Thermoactinomyces daqus CICC 10681 at different temperatures[J]. Food and Fermentation Industries, 2018
, 44(11)
: 49
-54
.
DOI: 10.13995/j.cnki.11-1802/ts.017038
[1] YAO S, LIU Y, ZHANG M J, et al. Thermoactinomyces daqus sp. nov. a thermophilic bacterium isolated from high temperature Daqu[J].International Journal of Systematic & Evolutionary Microbiology,2014,64(1):206-210.
[2] 姚粟,葛媛媛,李辉,等.利用非培养技术研究芝麻香型白酒高温大曲的细菌群落多样性[J].食品与发酵工业, 2012, 38(6):1-6.
[3] 马挺,刘如林.嗜热菌耐热机理的研究进展[J].微生物学通报, 2002, 29(2):86-89.
[4] 徐国恒.细胞膜的双层磷脂结构与功能[J].生物学通报, 2006, 41(9):11-14.
[5] 李颖.微生物生理学[M].北京:科学出版社, 2013.
[6] BLANKENBERG D, GORDON A, KUSTER G V, et al. Manipulation of FASTQ data with galaxy [J].Bioinformatics, 2010, 26(14):1 783-1 785.
[7] LANGMEAD B. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome[M]. Genome Biology,2009, 10(3):25-34.
[8] YAO S, XU Y, XIN C, et al. Genome Sequence of Thermoactinomyces daqus H-18,a novel thermophilic species isolated from high-temperature Daqu[J].Genome Announcements,2014,3(1):e01394-14.
[9] LI B, DEWEY C N. RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome[J].Bmc Bioinformatics, 2011, 12(1):323-325.
[10] LU G, LI Z, ZHANG X, et al. Expression analysis of lignin-associated genes in hard end pear (Pyrus pyrifolia, Whangkeumbae) and its response to calcium chloride treatment conditions[J].Journal of Plant Growth Regulation, 2015,34(2):251-262.
[11] 徐丽华.放线菌系统学[M].北京:科学出版社,2007.
[12] PATEL M S, ROCHE T E. Molecular biology and biochemistry of pyruvate dehydrogenase complexes[J]. Faseb Journal Official Publication of the Federation of American Societies for Experimental Biology, 1990, 4(14):3 224-3 233.
[13] 罗琦霞. Shewanella oneidensis脂肪酸生物合成及其调控的研究[D]. 杭州:浙江大学, 2015.
[14] KOGA Y. Thermal adaptation of the archaeal and bacterial lipid membranes.[J].Archaea-an International Microbiological Journal,2012, 2012(1):789-652.
[15] 蔡浩然.液晶态生物膜[J].科学通报,1978,23(4):209-216.
[16] 刘玮.液晶态生物膜结构及其相变同功能的关系[J].生物化学与生物物理进展,1985(1):18-24.
[17] HAZEL J R. Thermal adaptation in biological membranes: is homeoviscous adaptation the explanation?[J].Annual Review of Physiology,1995,57(1):19-42.
[18] AGUILAR P S, JR C J, De M D. A Bacillus subtilis gene induced by cold shock encodes a membrane phospholipid desaturase[J]. Journal of Bacteriology, 1998, 180(8):2 194-2 200.
[19] KANEDA T. Iso- and anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance[J]. Microbiological Reviews, 1991, 55(2):288-302.
[20] KANEDA T. Positional preference of fatty acids in phospholipids of Bacillus cereus and its relation to growth temperature[J]. Biochim Biophys Acta,1972,280(2):297-305.
