The effect of mannan-oligosaccharide (MOS) on high-fat diet (HFD) mice was studied. Four experimental groups were set as normal chow (NC), normal chow gavage with MOS (NC+MOS), high fat diet (HFD) and high fat diet gavage with MOS (HFD+MOS). The dosage of MOS was 6 g/kg body weight per day. After 11 weeks of experiment, mice were sacrificed and the gut microbiota of the mice at 11th week were detected by 16s high-throughput sequencing. LEfSe was used to identify the marker microorganisms. PICRUSt was used to predict the functional composition of gut microbiota, and gas chromatography was used to detect the concentrations of short-chain fatty acids (SCFAs) in cecal content. The results showed that MOS could significantly reduce the body weight gain of HFD mice. Supplement with MOS could not only change the structure of gut microbiota in both HFD and NC mice, but also selectively modulate some microbiome and increase the relative abundance of Akkermansia, Lactobacillus and Bifidobacterium etc. PICRUSt analysis showed that MOS enhanced lipid metabolism of gut microbiota in mice. Meanwhile, supplement with MOS increased the concentrations of acetic, propionic and butyric acid and decreased the concentration of valeric acid in cecal content of mice. The above results showed that MOS could not only help the weight-loss of HFD mice and modulate the gut microbiota structure, but also promote the proliferation of probiotics and enhance lipid metabolism function of gut microbiota with the increasing content of some SCFAs, Thus MOS had good probiotic effect on obese mice caused by high-fat diet.
WANG Hong-shan
,
ZHANG Xiao-juan
,
LI Heng
,
SHI Jin-song
,
XU Zheng-hong
. Prebiotic effects of mannan-oligosaccharide on high fat diet mice[J]. Food and Fermentation Industries, 2018
, 44(11)
: 63
-58
.
DOI: 10.13995/j.cnki.11-1802/ts.017193
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