Anthocyanins from colored rice were extracted with hydrochloric acid-methanol solutions under ultrasonic wave extraction. The detection of cyandin-3-O-glucoside chloride and peonidin-3-O-glucoside chloride in brown rice and rice bran were carried out by UHPLC-UV visible detector. The samples were four black rice, one purple rice and five red rice collected in Sichuan and Chongqing provinces. The results showed that the method was well linearly correlated in the concentration range of 0.5 to 50 μg/mL for anthocyanins (R2＞0.999); the recoveries of cyandin-3-O-glucoside chloride were 93.0%-98.5% with relative standard deviations (RSD) of 0.33%-3.50%; the recoveries of peonidin -3-O-glucoside chloride were 96.0%-111.7% with RSD of 0.42%-2.18%. The total amount of anthocyanins in black rice, black rice bran, purple rice and purple rice bran was 0-1 051.27, 74.06-206.82, 296.18 and 116.15 mg/kg respectively. Cyandin-3-O-glucoside chloride and peonidin-3-O-glucoside chloride were not detected in red rice, but cyandin-3-O-glucoside chloride was detected in some red rice bran samples, and the content was from 0 to 50.94 mg/kg.
ZHAO Shan
,
XI Qing-qing
,
LI Xi
,
LEI Xin-yu
,
ZHONG Ling-li
,
HUANG Shi-qun
,
LUO Ling
,
GUO Ling-an
,
ZHOU Hong
. Determination of anthocyanins in colored rice by ultra-high performance liquid chromatography[J]. Food and Fermentation Industries, 2018
, 44(11)
: 301
-306
.
DOI: 10.13995/j.cnki.11-1802/ts.016228
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