Metabolic pathway for synthesizing glucaric acid was constructed in Saccharomyces cerevisiae by co-expression of myo-inositol oxygenase encoded by miox4 from Arabidopsis thaliana and uronate dehydrogenase encoded by udh from Pseudomonas syringae. The yield of glucaric acid reached 3.8 g/L, the highest yield that had been ever reported in S. cerevisiae. This mehod could be widely used in metablic engineering of S. cerevisiae.
CHEN Na
,
LIN Lu-qi
,
MAO Yin
,
ZHAO Yun-ying
,
DENG Yu
. Metabolically engineered Saccharomyces cerevisiae for producing glucaric acid[J]. Food and Fermentation Industries, 2018
, 44(12)
: 15
-21
.
DOI: 10.13995/j.cnki.11-1802/ts.017492
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