Alginate oligosaccharides were prepared by hydrolyzing alginate with algae lyase from Isoptericola halotolerans CGMCC 5336. The components were analyzed by liquid chromatography-mass spectrometry and purified by gel chromatography. Four main components, named Fr1, Fr2, Fr3, and Fr4, were purified and identified. Their effects on the transcription and translation of low-density-lipoprotein receptor (LDLR) gene and pro-protein convertase subtilisin/kexin type 9 (PCSK9) were investigated. The results showed that Fr1 contained two oligosaccharides, pento- and hexo-oligosaccharides; Fr2, Fr3 and Fr4 were tetra-, tri- and di-oligosaccharides. Among them, Fr1, Fr3, and Fr4 significantly up-regulated the level of LDLR while Fr3 down-regulated the level of PCSK9, indicating that the up-regulated LDLR by Fr3 might be related to PCSK9. The up-regulation of LDLR by Fr4 was not related to PCSK9, however, might be regulated by the sterol response binding protein-2 (SREBP-2). These indicated that Fr3 and Fr4 may be further developed as new food additives for lowering the plasma cholesterol level.
CHEN Yan-jun
,
DOU Wen-fang
,
LI Heng
,
SHI Jin-song
,
XU Zheng-hong
. Isolation and lipoprotein regulation activity of alginate oligosaccharides[J]. Food and Fermentation Industries, 2018
, 44(12)
: 30
-35
.
DOI: 10.13995/j.cnki.11-1802/ts.017372
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