Isolation and lipoprotein regulation activity of alginate oligosaccharides

  • CHEN Yan-jun ,
  • DOU Wen-fang ,
  • LI Heng ,
  • SHI Jin-song ,
  • XU Zheng-hong
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  • 1(Key Laboratory of Carbohydrate Chemistry and Biotechnology, School of Pharmaceutical Science, Jiangnan University,Wuxi 214122, China)
    2(National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China)
    3(School of biotechnology, Jiangnan University, Wuxi 214122, China)

Received date: 2018-03-24

  Online published: 2019-01-22

Abstract

Alginate oligosaccharides were prepared by hydrolyzing alginate with algae lyase from Isoptericola halotolerans CGMCC 5336. The components were analyzed by liquid chromatography-mass spectrometry and purified by gel chromatography. Four main components, named Fr1, Fr2, Fr3, and Fr4, were purified and identified. Their effects on the transcription and translation of low-density-lipoprotein receptor (LDLR) gene and pro-protein convertase subtilisin/kexin type 9 (PCSK9) were investigated. The results showed that Fr1 contained two oligosaccharides, pento- and hexo-oligosaccharides; Fr2, Fr3 and Fr4 were tetra-, tri- and di-oligosaccharides. Among them, Fr1, Fr3, and Fr4 significantly up-regulated the level of LDLR while Fr3 down-regulated the level of PCSK9, indicating that the up-regulated LDLR by Fr3 might be related to PCSK9. The up-regulation of LDLR by Fr4 was not related to PCSK9, however, might be regulated by the sterol response binding protein-2 (SREBP-2). These indicated that Fr3 and Fr4 may be further developed as new food additives for lowering the plasma cholesterol level.

Cite this article

CHEN Yan-jun , DOU Wen-fang , LI Heng , SHI Jin-song , XU Zheng-hong . Isolation and lipoprotein regulation activity of alginate oligosaccharides[J]. Food and Fermentation Industries, 2018 , 44(12) : 30 -35 . DOI: 10.13995/j.cnki.11-1802/ts.017372

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