Polysaccharides were obtained from Pleurotus djamor by hot water extraction and alcohol precipitation. After removal of pigments and proteins by macroporous anion exchange resin D315 and Sevage method respectively, polysaccharides were purified by DEAE-cellulose-52 and Sephadex G-150. Molecular weight distribution and monosaccharide composition were analyzed by high performance gel filtration chromatography (HPGFC) and pre-column high performance liquid chromatography. The structures were preliminarily analyzed by ultraviolet, infrared spectrum and Congo red experiments. Their physicochemical properties were further determined. The results showed that three components were obtained with the molecular weights 12.9, 10.6, 2753.7 kDa respectively. PDP-1-1 was composed of mannose, glucose, galactose, Arabia sugar and fucose; PDP-2-1 was composed of mannose, glucose, galactose, xylose, Arabia sugar and fucose; PDP-3-1 was composed of mannose, glucose, galactose and fucose. Ultraviolet spectrum confirmed that the fractions were free of protein and nucleic acid and the infrared spectrum showed the characteristic absorption peak of polysaccharides. PDP-1-1 and PDP-3-1 had triple-helical structure.
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