In this paper, we mainly discussed the extraction process to extract the α-ketoglutarate in the fermentation broth.A new aqueous two-phase system contains Isopropanol and dipotassium hydrogen phosphate was adopted to extract α-ketoglutaric acid. Double water phase diagram was drawn by turbidity point method. Double beam ultraviolet-visible spectrophotometer was used for the qualitative and quantitative determination of alpha-ketoglutarate,to determine the best absorption wavelength for 235.14 nm, standard curve for C=1.823 92A+0.012 75(R2=0.999 23),in 0.005-0.25 g/L range had good linear relationship. In this experiment,the effects of isopropanol dosage, dipotassium hydrogen phosphate dosage and extraction temperature on α-ketoglutaric acid extraction were investigated.The optimum process was determined by single factor experiment and orthogonal experiment. The optimum conditions were as follows: the fermentation fluid total 10 g, isopropanol dosage is 6 g, dipotassium hydrogen phosphate dosage for 7 g,extraction temperature for 5 ℃. In this condition, the allocation coefficient of α-ketoglutaric acid was 3.215 5, and the extraction rate was 80.45%.
PEI Ying
,
SUN Yu-bo
,
HUANG Bin
. The extraction and separation of α-ketoglutarate by aqueous two-phase systems[J]. Food and Fermentation Industries, 2018
, 44(12)
: 150
-154
.
DOI: 10.13995/j.cnki.11-1802/ts.017150
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