A neutral Cordyceps militaris polysaccharide (CMP) from crude Cordyceps militaris polysaccharides was purified by using dicthylaminoethyl 52(DEAE-52)column chromatography. The CMP was characterized by ultraviolet-visible (UV-visible) and fourier transforming infrared spectroscopy (FT-IR). Gas chromatography-mass spectrometry (GC-MS) was applied to analyze the composition of monosaccharides. The weight-average molecular weight (MW) and the radii of gyration (Rg) of CMP were measured by multi-angle laser light scattering (MALLS), gel permeation chromatography (GPC) combined with reflective index detector (GPC-MALLS-RI). The chain conformation of CMP in solution was clarified at the same time. Additionally, the antioxidant activities of crude polysaccharides and CMP were compared. The results showed that CMP purified by DEAE-52 column had higher purity (mass fraction=76.63%), which was approximately 70.48% higher than that of crude polysaccharides (44.95%); The UV-visible spectrum showed that CMP had no significant absorption peaks at 260 nm and 280 nm. FT-IR revealed that CMP was an α-type polysaccharide. The monosaccharide compositions of CMP were mannose, glucose and galactose, with a molar ratio 1∶3.90∶1.51. The MW and Rg of CMP were 2.432×107 g/mol and 31 nm, respectively. Moreover, CMP exhibited a highly branched structure in solution. Furthermore, CMP had stronger DPPH and ABTS radical scavenging capacities than crude polysaccharides, which indicated that CMP possessed significant antioxidant activities.
JIANG Qi
,
LOU Zaixiang
,
WANG Zhengqi
,
WANG Hongxin
,
CHEN Xiaoyun
. Isolation and purification, structure and molecular conformation and antioxidant activities of polysaccharides from Cordyceps militaris[J]. Food and Fermentation Industries, 2019
, 45(1)
: 22
-28
.
DOI: 10.13995/j.cnki.11-1802/ts.017000
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