Using a promoter probe plasmid, promoter P28 was isolated from Bacillus amyloliquefaciens XH7, which exhibited β-Gal activity in Escherichia coli (2 350 Miller U/mL) and Bacillus subtilis (3 340 Miller U/mL). Sequence analysis showed that promoter P28 contained PctpA (a ctpA gene encoding carboxy-terminal processing protease) and Ptal (a tal gene encoding translaldolase) connection in series, and it was recognized by sigma factor σK. Thus, this study provided a new strong promoter to the promoter library of Bacillus species.
LIU Xiaochuan
,
CAI Xuxin
,
CHEN Huaqiang
. Clone and identification of a strong promoter from Bacillus amyloliquefaciens[J]. Food and Fermentation Industries, 2019
, 45(1)
: 41
-43
.
DOI: 10.13995/j.cnki.11-1802/ts.017857
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