Donkey-hide gelatin and bovine-hide gelatin were determined in 6 kinds of formulations, such as powder, capsule, lozenge, jell, and oral solution, and 67 samples of donkey-hide gelatin using UPLC-MS/MS. The contents of donkey-hide gelatin and bovine-hide gelatin were analysed and evaluated by measuring L-hydroxyproline, glycine, alanine, L-proline, moisture, and protein. The content of donkey-hide gelatin in tablets was found to be 39%-65%, and protein was 68%-84%. The detection rate of bovine-hide gelatin was 22.8%, and 2 batches exceeded the standard. The content of donkey-hide gelatin indicated as the sum of L-hydroxyproline, glycine, alanine, and L-proline had significant differences in other 5 formulations, as they were all lower than that in tablets. Moreover, 16 batches were detected to contain bovine-hide gelatin, but none of them exceeded the standard. The present method is repeatable and sensitive to distinguish donkey-hide gelatin and bovine-hide gelatin. It is necessary to pay attention to raw materials of donkey-hide gelatin and formulate standards for different formulations.
WANG Chao
,
CHI Shaoyun
,
YANG Zhao
,
ZHU Bo
,
PEI Lin
,
XU Hui
. Quality evaluation and determination of donkey-hide gelatin and bovine-hide gelatin in health-care food of donkey-hide gelatin[J]. Food and Fermentation Industries, 2019
, 45(1)
: 224
-228
.
DOI: 10.13995/j.cnki.11-1802/ts.016740
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