Quality evaluation and determination of donkey-hide gelatin and bovine-hide gelatin in health-care food of donkey-hide gelatin

  • WANG Chao ,
  • CHI Shaoyun ,
  • YANG Zhao ,
  • ZHU Bo ,
  • PEI Lin ,
  • XU Hui
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  • (Qingdao Institute for Food and Drug Control,Qingdao 266071,China)

Received date: 2018-01-08

  Online published: 2019-02-01

Abstract

Donkey-hide gelatin and bovine-hide gelatin were determined in 6 kinds of formulations, such as powder, capsule, lozenge, jell, and oral solution, and 67 samples of donkey-hide gelatin using UPLC-MS/MS. The contents of donkey-hide gelatin and bovine-hide gelatin were analysed and evaluated by measuring L-hydroxyproline, glycine, alanine, L-proline, moisture, and protein. The content of donkey-hide gelatin in tablets was found to be 39%-65%, and protein was 68%-84%. The detection rate of bovine-hide gelatin was 22.8%, and 2 batches exceeded the standard. The content of donkey-hide gelatin indicated as the sum of L-hydroxyproline, glycine, alanine, and L-proline had significant differences in other 5 formulations, as they were all lower than that in tablets. Moreover, 16 batches were detected to contain bovine-hide gelatin, but none of them exceeded the standard. The present method is repeatable and sensitive to distinguish donkey-hide gelatin and bovine-hide gelatin. It is necessary to pay attention to raw materials of donkey-hide gelatin and formulate standards for different formulations.

Cite this article

WANG Chao , CHI Shaoyun , YANG Zhao , ZHU Bo , PEI Lin , XU Hui . Quality evaluation and determination of donkey-hide gelatin and bovine-hide gelatin in health-care food of donkey-hide gelatin[J]. Food and Fermentation Industries, 2019 , 45(1) : 224 -228 . DOI: 10.13995/j.cnki.11-1802/ts.016740

References

[1] 吴长虹,王若光.阿胶的历史沿革、研究现状及相关思考[J].湖南中医药大学学报,2008,28(6):77-79.
[2] 胡军影,呈显隆,肖新月,等.阿胶的化学成分及质量评价方法研究进展[J].中国药事,2007,21(3):193-195.
[3] 赵曦,翟乙娟,都恒青,等.30种商品阿胶的质量比较[J].中国药学杂志,2000,35(10):690-692.
[4] 高景会,王蕊,范锋.阿胶现代研究进展[J].中国药事,2011,25(4):396-401.
[5] 尚汝瑶,杨昭鹏,冯磊.国家药品补充检验方法管理工作的现状与思考[J].中国药事,2014,28(6):576-579.
[6] 陈慧慧,冯明建,朱海芳,等.阿胶药理研究进展[J].药物研究,2014,31(1):23-26.
[7] 王若光,尤昭玲,刘小丽,等.基于激光解析/离子化-飞行时间质谱技术的中药阿胶蛋白质组分析[J]. 中国组织工程研究与临床康复,2007,13:2 518-2 521.
[8] 吴亚君,王斌,刘鸣畅,等.阿胶中马和驴成分的实时荧光PCR检测[J].食品科学,2014,35(8):85-88.
[9] 张贵锋,刘涛,王前,等.中药阿胶的质量控制方法研究[J]. 药物生物技术,2009,3:250-254.
[10] 国家药典委员会.中华人民共和国药典[M].一部.北京:化学工业出版社,2015:189-190.
[11] 中国食品药品检定研究院.阿胶中牛皮源质量的补充检验方法[EB/OL].[2014-12-21].http://www.doc88.com/p-3137587400245.html.
[12] 国家药典委员会.中华人民共和国药典[M].四部.北京:化学工业出版社,2015:103-104.
[13] GB 5009.5—2010,食品安全国家标准食品中蛋白质的测定[S].北京:中华人民共和国卫生部,2010.
[14] 毛跟年,郭倩,瞿建波,等.阿胶化学成分及药理作用研究进展[J].动物医学进展,2010,31(11):83-85.
[15] 汪学德,崔英德,刘兵戈,等.芝麻各成分相关性分析[J].中国油脂,2015,40(11):99-103.
[16] 陆俊,赵安琪,成策,等.核桃营养成分与生理活性及开发利用[J].食品与机械,2014,30(6):238-242.
[17] 王蓉珍,赵子青,林勤保,等.大枣功效成分检测的研究进展[J].食品工业科技,2012,33(4):423-426.
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