With the development of fermentation and sensor technology, the amount of fermentation data from laboratory and fermentation industries is increasing quickly. How to effectively manage and utilize these data has become one of the puzzling problems for technicians. In order to solve this problem, the “Bai Lun Hui Shen” fermentation data collection/analysis system (Bioplot) was designed by using C#2010. The Bioplot software collected data through reserved general communication interface, including DDE, OPC, and TCP. Additionally, it managed data using MySQL database and provided the users with functions of data summary, data table display, and comparing multi-batch and multi-parameter curves flexibly. The Bioplot software was used to connect two bioreactors produced by different manufacturers, and its effectiveness and universality during the real fermentation process were validated. The results indicated that all the functional modules contained in Bioplot could run normally and show the desired effects.