Streptomyces diastatochromogenes cell protein isolates were dried by nitrogen-purge, oven-drying and vacuum lyophilization, respectively. The amino acid components of the cell protein isolates were analyzed by gas chromatography and mass spectrometry (GC-MS). The results showed that 16 kinds of amino acids could only be detected with vacuum lyophilization, indicating the vacuum lyophilization was a suitable method for preparing protein-bound amino acids from S. diastatochromogenes cell protein isolates for 13C-labeled metabolic flux analysis during ε-poly-L-lysine biosynthesis.
GUO Fengzhu
,
TAN Zhilei
,
SHI Yifei
,
SONG Fu
,
ZHONG Qiding
,
JIA Shiru
. Effects of different drying methods on cell protein isolate and its amino acid components of Streptomyces diastatochromogenes[J]. Food and Fermentation Industries, 2019
, 45(2)
: 113
-117
.
DOI: 10.13995/j.cnki.11-1802/ts.017988
[1] NISHIKAWA M,OGAWA K.Distribution of microbes producing antimicrobial ε-poly-L-lysine polymers in soil microflora determined by a novel method[J] .Applied and Environmental Microbiology,2002,68(7):3575-3581.
[2] EL-SERSY N A,ABDELWAHAB A E,ABOUELKHIIR S S,et al.Antibacterial and anticancer activity of ε-poly-L-lysine(ε-PL) produced by a marine Bacillus subtilis sp.[J] .Journal of Basic Microbiology,2012,52(5):513-522.
[3] SHUKLA S C, SINGH A,PANDEY A K,et al.Review on production and medical applications of ε-polylysine[J] .Biochemical Engineering Journal,2012,65:70-81.
[4] HAMANO Y.Amino-acid Homopolymers Occurring in Nature[M] . Berlin Heidelberg:Springer,2010:61-75.
[5] HIRAKI J,ICHIKAWA T,NINOMIYA S,et al.Use of ADME studies to confirm the safety of ε-polylysine as a preservative in food[J] .Regulatory Toxicology and Pharmacology,2003,37(2):328-340.
[6] BO Tao,LIU Miao,ZHONG Cheng,et al.Metabolomic analysis of antimicrobial mechanisms of ε-poly-L-lysine on Saccharomyces cerevisiae[J] .Journal of Agricultural and Food Chemistry,2014,62(19):4454-4465.
[7] BO Tao,HAN Pei-pei,SU Qin-zhi,et al.Antimicrobial ε-poly-L-lysine induced changes in cell membrane compositions and properties of Saccharomyces cerevisiae[J] .Food Control,2016,61:123-134.
[8] HYLDGAARD M,MYGIND T,VAD B S,et al.The antimicrobial mechanism of action of ε-poly-L-lysine[J] .Applied and Environmental Microbiology,2014,80(24):7758-7770.
[9] 宋庆超,李秀雯,王洪翠,等.ε-聚赖氨酸与纳他霉素复配剂抑菌性能的研究[J] .中国酿造,2012,31(7):80-83.
[10] YOSHIDA T,NAGASAWA T.ε-Poly-L-lysine:microbial production,biodegradation and application potential[J] .Applied Microbiology and Biotechnology,2003,62(1): 21-26.
[11] RAJAPAKSHA D S W,KODITHUWAKKU K A H T,SILVA K F S T,et al.Evaluation of potassium sorbate and ε-polylysine for their inhibitory activity on post-acidification of set yoghurt under cold storage for 20 days[J] .International Journal of Scientific and Research Publications,2013,3:1-7.
[12] 张雪,赵娟,谭之磊,等.生物食品添加剂在草莓保鲜中的应用[J] .中国食品添加剂,2013(3):158-162.
[13] 贾士儒,谭之磊,罗晶,等.一种简单快速、货架期长的馒头的制作方法[P] .中国专利:ZL 201310182022.X,2014-07-14.
[14] MIYA S,TAKAHASHI H,HASHIMOTO M,et al.Development of a controlling method for Escherichia coli O157:H7and Salmonella spp. in fresh market beef by using polylysine and modified atmosphere packaging[J] .Food Control,2014,37(1):62-67.
[15] HATA T,SATO T,ICHIKAWA T,et al.Antifungal activity of protamine salmine hydrochloride and ε-poly-L-lysine in actual food systems,rice or wheat-based confectioneries[J] .Journal of Food Processing and Preservation,2016,40(6):1180-1187.
[16] ZHU Hui-xia,JIA Shi-ru,YANG Hong-jiang,et al.Characterization of bacteriostatic sausage casing:A composite of bacterial cellulose embedded with ε-polylysine[J] .Food Science and Biotechnology,2010,19(6):1479-1484.
[17] PANDEY A K,KUMAR A.Improved microbial biosynthesis strategies and multifarious applications of the natural biopolymer ε-poly-L-lysine[J] .Process Biochemistry,2014,49(3):496-505.
[18] 关于批准ε-聚赖氨酸等4种食品添加剂新品种等的公告(2014年第5号)[EB/OL] .中华人民共和国国家卫生和计划生育委员会,2014:213-221.
[19] 贾士儒.生物防腐剂[M] .北京:中国轻工业出版社,2009.
[20] WIECHERT W.13C metabolic flux analysis[J] .Metabolic Engineering,2001,3(3):195-206.
[21] 钟其顶,李国辉,王道兵,等.气相色谱-燃烧-同位素质谱仪(GC-C-IRMS)测定游离氨基酸的δ13C值[J] .酿酒科技,2013(9):7-10.
[22] 申铁.13C代谢通量分析平台的建立与改良[D] .合肥:中国科学技术大学,2010.
[23] ZAMBONI N,FENDT S,RÜHL M,et al.13C-based metabolic flux analysis[J] .Nature Protocols,2009,4(6):878-892.
[24] 赵东东,钟其顶,李国辉,等.低丰度13C标记的酿酒酵母代谢通量分析探讨研究[J] .酿酒科技,2014(3):13-16.
[25] 石召华,黄文芳,陈立军,等.不同干燥方式对地龙提取物中17种氨基酸的影响[J] .中成药,2015,37(5):1135-1138.
[26] QUEK L E,DIETMAIR S,KRÖMER J,et al.Metabolic flux analysis in mammalian cell culture[J] .Metabolic Engineering,2010,12(2):161-171.