Streptomyces diastatochromogenes cell protein isolates were dried by nitrogen-purge, oven-drying and vacuum lyophilization, respectively. The amino acid components of the cell protein isolates were analyzed by gas chromatography and mass spectrometry (GC-MS). The results showed that 16 kinds of amino acids could only be detected with vacuum lyophilization, indicating the vacuum lyophilization was a suitable method for preparing protein-bound amino acids from S. diastatochromogenes cell protein isolates for 13C-labeled metabolic flux analysis during ε-poly-L-lysine biosynthesis.
GUO Fengzhu
,
TAN Zhilei
,
SHI Yifei
,
SONG Fu
,
ZHONG Qiding
,
JIA Shiru
. Effects of different drying methods on cell protein isolate and its amino acid components of Streptomyces diastatochromogenes[J]. Food and Fermentation Industries, 2019
, 45(2)
: 113
-117
.
DOI: 10.13995/j.cnki.11-1802/ts.017988
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