Established a method for detecting the 7 short-chain fatty acids(formic acid, acetic acid, propanoic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid)in the fermented milk by gas chromatography (GC). The samples were pretreated by derivation, separated by chromatographic column, qualitative analysis by retention time and quantitative analysis by peak area. All the linear ranges of 7 short-chain fatty acids were 20-2 000 mg/L, the limits of quantitation were 20 mg/L, the limits of detection were 5 mg/L, the correlation coefficients(R2)were greater than 0.999, the recovery range was 93.57%-108.20%, the relative standard deviation (RSD) range of the results was 2.40%-9.56%. The method is accurate, sensitive and reproducible and can be used to detect the content of short-chain fatty acids in the fermented milk.
DING Yan
,
WANG Juan
,
ZHANG Di
. Determination of 7 short-chain fatty acids in fermented milk by gas chromatography[J]. Food and Fermentation Industries, 2019
, 45(2)
: 202
-206
.
DOI: 10.13995/j.cnki.11-1802/ts.018082
[1] 农业大词典编辑委员会.农业大词典[M] .北京:中国农业出版社,1998:1948.
[2] 王林云.养猪词典[M] .北京:中国农业出版社,2004:208.
[3] 中国营养学会.营养科学词典[M] .北京:中国轻工业出版社,2013:357.
[4] 贾益群,叶福媛,王双,等.生物样品中短链脂肪酸的快速提取与分析方法[J] .实验室研究与探索, 2012,31(7):262-264.
[5] 陈永福,王记成,云振宇,等.高效液相色谱法测定传统发酵乳中的有机酸组成[J] .中国乳品工业,2007,35(1):54-58.
[6] 李咏如,李徐,陶胜男,等.反相高效液相色谱法测定Rancimat测量池水中的短链脂肪酸[J] .中国油脂,2017,42(9):126-128.
[7] 郭宏,刘广福,王风娇,等.酸菜发酵有机酸反相高效液相色谱法分析[J] .中国公共卫生,2015,31(12):1632-1634.
[8] 郭子好,方华,夏志生,等.反相高效液相色谱法测定发酵豆粕中的乳酸含量[J] .饲料工业,2014(S1):121-123.
[9] 郑志,姜绍通,潘丽军,等.反相高效液相色谱法测定发酵液中乳酸的含量[J] .食品科学,2003,24(12):89-91.
[10] 张浩勤,张伟,张翔,等.气相色谱法测定牛粪厌氧发酵液中挥发性脂肪酸[J] .郑州大学学报(工学版),2007,28(2):51-53.
[11] 张树成,张海兰,王维林,等.功能性便秘儿童粪便短链脂肪酸的气相色谱分析及临床意义[J] .中华小儿外科杂志,2010,31(4):276-279.
[12] 赵晓亚,王跃飞,杨帆,等.气相色谱法快速分析人唾液中7种短链脂肪酸[J] .分析化学,2016,44(7):1009-1014.
[13] 耿梅梅,许丽卫,袁红朝,等.气相色谱法测定猪结肠内容物中短链脂肪酸含量[J] .现代生物医学进展,2015,15(6):1010-1014.
[14] 谭力,罗楠,刘放南,等.非衍生化-气相色谱法测定人血清游离脂肪酸谱[J] .肠外与肠内营养,2003,10(4):236-238.
[15] 庞锦伟,姚欣,雷超,等.气相色谱-质谱法测定酵素产品中短链脂肪酸[J] .中国酿造,2017,36(8):165-167.
[16] FURUHASHI T, SUGITATE K,NAKAI T,et al.Rapid profiling method for mammalian feces short chain fatty acids by GC-MS[J] .Analytical Biochemistry,2017, 543: 51-54.
[17] 江振作,王跃飞,陈荣荣,等.顶空气相色谱-质谱联用法分析粪便中挥发性脂肪酸[J] .分析化学,2014,42(3):429-435.
[18] 赵晓亚,江振作,王跃飞.人的粪便、血浆、唾液、呼出气体中短链脂肪酸的分析[J] .中国微生态学杂志,2015,27(2):130-134.
[19] 招云芳,陆舍铭,王海利,等.顶空气相色谱质谱法快速测定液体食品中的挥发性酸[J] .分析测试学报,2009,28(12):1456-1459.
[20] 米智慧,陈永福,张和平.超高效液相色谱质谱联用技术在发酵乳有机酸分析中的应用[J] .中国乳品工业,2017,45(12):37-39.
[21] 谭力,鞠熀先,黎介寿.生物样品中短链脂肪酸的提取与测定[J] .色谱,2006,24(1): 81-87.
[22] 关家锐,邓丛蕊,王吉顺,等.衍生化反相高效液相色谱测定葡萄酒中有机酸的研究[J] .色谱,1993,11(5):282-285.
