Isolation and identification of histamine-producing bacteria in saltedChinese herring

  • WU Jiajia ,
  • WANG Siqi ,
  • DAI Zhiyuan
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  • 1(Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China)
    2(College of Life Science, China Jiliang University, Hangzhou 310018, China)

Received date: 2018-06-27

  Online published: 2019-03-11

Abstract

Accumulation of biogenic amines in fermented seafood products caused by bacterial activity is a serious food safety problem. This study used selective plates to screen histamine-producing bacteria from traditional salted Chinese herring that were collected from different fermentation periods during its production. The isolates were identified by 16S rDNA sequencing. Histidine decarboxylase genes were amplified using polymerase chain reaction (PCR), while the histamine content in the culture supernatant was detected using high performance liquid chromatography (HPLC). The results indicated that thirty-two presumptive histamine-producing isolates were obtained, which were classified into three genera and eight species. Staphylococcus was the predominant genus (twenty-nine strains), with S. saprophyticus was the dominant species (sixteen strains). Histidine decarboxylase genes were detected among twenty-four isolates, and different concentrations of histamine were detected in the cultures of twenty-three isolates. The average content of histamine produced was 38.10 μg/mL. Among which, one S. saprophyticus strain produced the highest histamine of 100.21 μg/mL. Studying histamine-producing bacteria in salted Chinese herring during its production has theoretical significances in evaluating the safety of salted Chinese herring as well as guiding the selection of fermentation strains scientifically.

Cite this article

WU Jiajia , WANG Siqi , DAI Zhiyuan . Isolation and identification of histamine-producing bacteria in saltedChinese herring[J]. Food and Fermentation Industries, 2019 , 45(3) : 71 -76 . DOI: 10.13995/j.cnki.11-1802/ts.018141

References

[1] GULLAUME D, GAELLE I, CHARLOTTE H, et al. Validation of standard method EN ISO 19343 for the detection and quantification of histamine in fish and fishery products using high-performance liquid chromatography[J].International Journal of Food Microbiology, 2019, 288(2): 97-101.
[2] MARUŠI? N, VIDACˇEK S, JANCˇI T, et al. Determination of volatile compounds and quality parameters of traditional Istrian dry-cured ham[J]. Meat Science, 2014, 96(4):1 409.
[3] 欧昌荣,管娟,汤海青,等. 鲭鱼中组胺降解菌的筛选鉴定和发酵条件初探[J]. 中国食品学报, 2014, 14(8):158-164.
[4] CZAJKOWSKA M A, LESZCAYNSKA J. Risk assessment related to biogenic amines occurrence in ready-to-eat baby foods[J]. Food & Chemical Toxicology, 2017, 105:82-92.
[5] HSIUHUA H, TINCHEN C, LIN H C, et al. Histamine content and histamine-forming bacteria in dried milkfish (Chanos chanos) products[J]. Food Chemistry, 2009, 114(3):933-938.
[6] TAKHASHI H, KIMURA B, YOSHIKAWA M, et al. Cloning and sequencing of the histidine decarboxylase genes of gram-negative, histamine-producing bacteria and their application in detection and identification of these organisms in fish[J]. Applied & Environmental Microbiology, 2003, 69(5):2 568-2 579.
[7] JEONG D W, LEE B, HER J Y, et al. Safety and technological characterization of coagulase-negative staphylococci isolates from traditional Korean fermented soybean foods for starter development[J]. International Journal of Food Microbiology, 2016, 236:9-16.
[8] KORAL S, TUFAN B, SCAVNICAR A, et al. Investigation of the contents of biogenic amines and some food safety parameters of various commercially salted fish products[J]. Food Control, 2013, 32(2):597-606.
[9] RABIE M, SIMON S L, SILIHA H, et al. Changes in free amino acids and biogenic amines of Egyptian salted-fermented fish (Feseekh) during ripening and storage[J]. Food Chemistry, 2009, 115(2):635-638.
[10] ZHANG He, LI Yan, XU Kunhua, et al. Microbiological changes and biodiversity of cultivable indigenous bacteria in sanbao larger yellow croaker (Pseudosciaena crocea), a Chinese salted and fermented seafood[J]. Journal of Food Science, 2015, 80(4):M776-M781.
[11] 郝淑贤,魏涯,周婉君,等. 鲭鱼生物胺生成菌的分离与鉴定[J]. 食品科学, 2015, 36(7):97-100.
[12] 袁开,吴佳佳,朱诚,等. 养殖鱼塘底泥微生物抗生素耐药基因分布分析[J]. 环境科学学报, 2017, 37(10):3 649-3 655.
[13] JAW Y M, CHEN Y Y, LEE Y C, et al. Histamine content and isolation of histamine-forming bacteria in fish meal and fish soluble concentrate[J]. Fisheries Science, 2012, 78(1):155-162.
[14] 杨健,吴祖芳,周秀锦,等. 冷冻鲣鱼中产组胺菌的分离筛选及其生物学特性研究[J]. 中国食品学报, 2012, 12(8):25-31.
[15] ANGENLIKI S D, PANAGIOTA F, STEFAAN D S, et al. Effect of temperature and pH on the community dynamics of coagulase-negative staphylococci during spontaneous meat fermentation in a model system[J]. Food Microbiology, 2018,76:180-188.
[16] JANSSENS M, MIJNSBRUGGE A V D, MAINAR M S, et al. The use of nucleosides and arginine as alternative energy sources by coagulase-negative staphylococci in view of meat fermentation[J]. Food Microbiology, 2014, 39(5):53-60.
[17] SEITTER M, NERZ C, ROSENSTEIN R, et al. DNA microarray based detection of genes involved in safety and technologically relevant properties of food associated coagulase-negative staphylococci[J]. International Journal of Food Microbiology, 2011, 145(2-3):449-458.
[18] FUKAMI K, SATOMI M, FUNATSU Y, et al. Characterization and distribution of Staphylococcus sp. implicated for improvement of fish sauce odor[J]. Fisheries Science, 2004, 70(5):916-923.
[19] FENG Yarong. Significance of biogenic amines to food safety and human health [J]. Meat Research, 2005, 29(7):675-690.
[20] 王颖,邱璠,韩北忠,等. 食品中的生物胺及其检测方法[J]. 中国酿造, 2011, 30(10): 1-5.
[21] COTON E, COTON M. Evidence of horizontal transfer as origin of strain to strain variation of the tyramine production trait in Lactobacillus brevis[J]. Food Microbiology, 2009, 26(1):52-57.
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