Differentiating transcriptomic patterns and functional analysis of Hansenula anomala during cultivation

  • TANG Chao ,
  • ZHANG Hanyu ,
  • WANG Ting ,
  • FENG Guangwen ,
  • QIAN Weidong ,
  • CAI Changlong ,
  • MAO Peihong
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  • 1 (Research Center of Rodiation Ecology and Ion Beam Biotechnology, College of Physics Science and Technology, Xinjiang University, Urumqi 830046, China)
    2 (School of Food and Biological Engineering, Shaanxi University of Science & Technology,Xi'an 710021, China)
    3 (Research Center of Ion Beam Biotechnology and Biodiversity, Xi'an Technological University, Xi'an 710032, China)

Received date: 2018-09-05

  Online published: 2019-03-26

Abstract

Hansenula anomala has outstanding performances on fermentation, esterification, and accumulating tryptophan. To illustrate the relationship between genes expression and metabolism of H. anomala during fermentation, differentially expressed genes (DEGs) at different fermentation points (0, 24, 48, 72, 96 h) were analyzed by using transcriptomic sequencing and bioinformatic methods. The results showed that 585, 487, 154, and 615 genes were up-regulated at 24, 48, 72, 96 h, respectively, while 1112, 725, 5, and 245 genes were down-regulated. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment of DEGs indicated that the metabolic activity of H. anomala decreased from 0 h to 48 h, but increased from 48 h to 96 h. Additionally, H. anomala had strong metabolic activity and genetic information processing ability at 96 h. The differential gene expression matrix analysis of the tryptophan pathway showed that the synthesis rate of tryptophan in H. anomala decreased from 0 h to 24 h and increased thereafter, while the decomposition rate of tryptophan increased from 0 h to 48 h, and then decreased. In summary, these results provides a theoretical basis for molecular breeding and metabolic regulation of H. anomala.

Cite this article

TANG Chao , ZHANG Hanyu , WANG Ting , FENG Guangwen , QIAN Weidong , CAI Changlong , MAO Peihong . Differentiating transcriptomic patterns and functional analysis of Hansenula anomala during cultivation[J]. Food and Fermentation Industries, 2019 , 45(4) : 1 -6 . DOI: 10.13995/j.cnki.11-1802/ts.018697

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