Hansenula anomala has outstanding performances on fermentation, esterification, and accumulating tryptophan. To illustrate the relationship between genes expression and metabolism of H. anomala during fermentation, differentially expressed genes (DEGs) at different fermentation points (0, 24, 48, 72, 96 h) were analyzed by using transcriptomic sequencing and bioinformatic methods. The results showed that 585, 487, 154, and 615 genes were up-regulated at 24, 48, 72, 96 h, respectively, while 1112, 725, 5, and 245 genes were down-regulated. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment of DEGs indicated that the metabolic activity of H. anomala decreased from 0 h to 48 h, but increased from 48 h to 96 h. Additionally, H. anomala had strong metabolic activity and genetic information processing ability at 96 h. The differential gene expression matrix analysis of the tryptophan pathway showed that the synthesis rate of tryptophan in H. anomala decreased from 0 h to 24 h and increased thereafter, while the decomposition rate of tryptophan increased from 0 h to 48 h, and then decreased. In summary, these results provides a theoretical basis for molecular breeding and metabolic regulation of H. anomala.
TANG Chao
,
ZHANG Hanyu
,
WANG Ting
,
FENG Guangwen
,
QIAN Weidong
,
CAI Changlong
,
MAO Peihong
. Differentiating transcriptomic patterns and functional analysis of Hansenula anomala during cultivation[J]. Food and Fermentation Industries, 2019
, 45(4)
: 1
-6
.
DOI: 10.13995/j.cnki.11-1802/ts.018697
[1] COMITINI F, GOBBI M, DOMIZIO P, et al. Selected non-Saccharomyces wine yeasts in controlled multistarter fermentations with Saccharomyces cerevisiae [J]. Food Microbiology, 2011, 28(5): 873-882.
[2] LEE P R, CHONG I S M, YU B, et al. Effects of sequentially inoculated Williopsis saturnus and Saccharomyces cerevisiae on volatile profiles of papaya wine [J]. Food Research International, 2012, 45(1): 177-183.
[3] 刘景,王欣,辛红鸿,等. 非酿酒酵母的筛选及其发酵低醇苹果汁的研究[J]. 中国食品学报, 2017, 17(1): 134-140.
[4] EBIHARA Y, NIITSU N, TERUI G. Fermentative production of tryptophan from indole by Hansenula anomala[J]. Ferment Technol, 1969, 47(12): 733-738.
[5] WAKASA K, ISHIHARA A. Metabolic engineering of the tryptophan and phenylalanine biosynthetic pathways in rice [J]. Plant Biotechnology, 2009, 26(5): 523-533.
[6] 毛培宏,吕杰. 离子束重组酵母菌Ar_Han0458的RAPD与SSH的初步研究[J]. 基因组学与应用生物学, 2015, 34(3): 449-453.
[7] LU Jie, JIN Xiang, MAO Pei-hong, et al. Transfer of Ephedra genomic DNA to yeasts by ion implantation [J]. Appl Biochem Biotechnol, 2009, 158(3): 571~581
[8] WANG Z, GERSTEIN M, SNYDER M. RNA-Seq: A revolutionary tool for transcriptomics [J]. Nature reviews genetics, 2009, 10(1): 57.
[9] BURKE D, DAWSON D, STEARNS T. Methods in yeast genetics: A cold spring harbor laboratory course manual [M]. New York: Cold Spring Harbor Lab Press, 2000: 171-172.
[10] SAHRAEIAN S M E, MOHIYUDDIN M, SEBRA R, et al. Gaining comprehensive biological insight into the transcriptome by performing a broad-spectrum RNA-seq analysis [J]. Nature communications, 2017, 8(1): 59.
[11] KIM D, LANGMEAD B, SALZBERG S L. HISAT: A fast spliced aligner with low memory requirements [J]. Nature methods, 2015, 12(4): 357.
[12] ANDERS S, PYL P T, HUBER W. HTSeq-a Python framework to work with high-throughput sequencing data [J]. Bioinformatics, 2015, 31(2): 166-169.
[13] GÖTZ S, ARNOLD R, SEBASTIÁN-LEÓN P, et al. B2G-FAR, a species-centered GO annotation repository [J]. Bioinformatics, 2011, 27(7):919-924.
[14] MORIYA Y, ITOH M, OKUDA S, et al. KAAS: An automatic genome annotation and pathway reconstruction server [J]. Nucleic acids research, 2007, 35:W182-W185.
[15] LOVE M I, HUBER W, ANDERS S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 [J]. Genome biology, 2014, 15(12): 550.
[16] YOUNG M D, WAKEFIELD M J, SMYTH G K, et al. Gene ontology analysis for RNA-seq: Accounting for selection bias [J]. Genome biology, 2010, 11(2): R14.
[17] MALDONADO E N, LEMASTERS J J. ATP/ADP ratio, the missed connection between mitochondria and the Warburg effect[J]. Mitochondrion, 2014, 19: 78-84.
[18] WEI San-hua, LIN Fang, WANG Xi, et al. Prognostic significance of stathmin expression in correlation with metastasis and clinicopathological characteristics in human ovarian carcinoma[J]. Acta Histochemica, 2008, 110(1): 59-65.
[19] BRANDÃO I D S L, OLIVEIRA-MORAES H M D S, SOUZA M C M D, et al. Elastin increases biofilm and extracellular matrix production of Aspergillus fumigatus[J]. Brazilian Journal of Microbiology, 2018, 49(3): 675-682.
[20] 李玲阁,唐任天,傅妙福,等.色氨酸发酵的初步研究[J].微生物学报, 1975, 15(3): 212-216.
