The pre-rehydrated vacuum-packaged Dougan is rich in nutrients and high in moisture, however, it has short shelf-life due to spoilage mainly by Bacillus spores. To effectively prevent spoilage, nisin, ε-polylysine, and potassium sorbate on four dominant spoilage Bacillus strains were tested, and their minimal inhibitory concentrations and inhibition rates were examined. The effects of recombinant preservatives were also comparatively evaluated under different utility conditions. The results showed that nisin and ε-polylysine had stronger antibacterial activities against four strains than that of potassium sorbate. The optimal formulation of recombinant preservatives determined by response surface methodology was 22.50 μg/mL nisin, 57.54 μg/mL ε-polylysine, and 425 μg/mL potassium sorbate. The maximal antibacterial and preservative effects of recombinant preservatives were obtained together with heat treatment at 100 ℃ for 20 min. The shelf-life of treated pre-rehydrated Dougan extended up to 10 days at 25 ℃, which was 4 days longer than the control. This study provides references for preserving pre-rehydrated vacuum-packaged Dougan.
TANG Guiyu
,
TANG Yilin
,
DAI Boren
,
JIANG Ailing
,
ZHANG Zhiqing
,
SHEN Guanghui
. Development of preservatives for anticorrosion of pre-rehydrated vacuum-packed Dougan[J]. Food and Fermentation Industries, 2019
, 45(4)
: 110
-116
.
DOI: 10.13995/j.cnki.11-1802/ts.018641
[1] 汪立平,张庆华,赵勇,等. 变质豆浆中腐败微生物的分离与初步鉴定[J]. 微生物学通报, 2007, 34(4): 621-624.
[2] 郑丽君,申光辉,张志清,等. 真空包装免泡豆杆优势腐败细菌分离鉴定及其致腐能力分析[J]. 食品科学, 2018, 39(2): 177-184.
[3] 鲁海波,张芳,王龙祥,等. 天然防腐剂在豆腐中的应用研究[J]. 中国粮油学报, 2011, 26(9): 29-32.
[4] 唐仁勇,刘达玉,郭秀兰,等. 乳酸链球菌素及其在肉制品中的应用[J]. 成都大学学报(自然科学版), 2010, 29(1): 14-17.
[5] CHHEDA A H, VERNEKAR M R. A natural preservative ε-poly-L-lysine: fermentative production and applications in food industry[J]. International Food Research Journal, 2015, 22(1): 23-30.
[6] 任剑豪,易灵,吴卫国. 山梨酸及其盐类防腐作用的研究现状及发展趋势[J]. 农产品加工, 2017(17): 48-50; 55.
[7] 郑丽君,申光辉,张志清,等. 免泡豆杆优势腐败菌腐败能力及产生物胺特性分析[J]. 食品与发酵工业, 2017, 43(10): 56-62.
[8] 国家卫生和计划生育委员会. GB 2760—2014食品安全国家标准食品添加剂使用标准[S]. 北京:中国标准出版社, 2014.
[9] 杨晓韬,李春,周晓宏. 7种食品防腐剂对肉制品污染微生物的抑菌效果比较研究[J]. 食品科学, 2012, 33(11): 12-16.
[10] 国家卫生和计划生育委员会. GB 5009. 228—2016食品安全国家标准食品中挥发性盐基氮的测定[S]. 北京:中国标准出版社, 2016.
[11] 卜宇芳,李文强,谢灵来,等. 休闲豆腐干贮藏过程中品质变化研究[J]. 食品与机械, 2016, 32(2): 115-118.
[12] WANGH, LIU X C, ZHANG Y M, et al. Spoilage potential of three different bacteria isolated from spoiled grass carp (Ctenopharyngodo nidellus) fillets during storage at 4 ℃[J]. LWT - Food Science and Technology, 2017, 81: 10-17.
[13] 王敏. 非发酵性豆制品(豆腐丝)主要腐败细菌的分离鉴定及其防腐研究[D]. 保定:河北农业大学, 2004.
[14] WANG G Y, WANG H H, HAN Y W, et al. Evaluation of the spoilage potential of bacteria isolated from chilled chicken in vitro and in situ[J]. Food Microbiology, 2017, 63: 139-146.
[15] 王敏,路玲玲,朱会霞,等. 蜂胶用于非发酵豆制品保鲜的条件优化[J]. 中国酿造, 2008(14): 62-63, 120.
[16] 王伟,耿飞,胡筱,等. 甘草提取液对大肠杆菌O157:H7抑菌作用及豆腐干的保鲜研究[J]. 中国食品学报, 2012, 12(1): 105-111.
[17] 李苗云,郝红涛,赵改名,等. 高温火腿肠在贮藏过程中质构稳定性研究[J]. 食品科学, 2010, 31(22): 473-476.
[18] BOURNE M C. Food Texture and Viseosity[M]. 2nd Edition. England: Aeademie Press, 2002: 55-56.