Enterotoxigenic Escherichia coli (ETEC) is a leading bacterial cause of diarrhea in weaned piglets. An effective prevention and treatement of ETEC caused diarrhea is to isolate a potential probiotic Bacillus strain which could produce exopolysaccharide (EPS) with anti-hemagglutination activity towards ETEC. Bacillus amyloliquefaciens JN4 was isolated from healthy piglets faeces. When cultured with LB medium containing 100 g/L sucrose, it could produce more than 20 g/L EPS. The EPS showed anti-hemagglutination activity with a titer of 4 mg/mL, lower than that of EPS from other Bacillus strains. The spores of JN4 were tolerant to gastric and small intestinal juice, indicated its capable to survive in the intestine, and the JN4 strain was sensitive to most commercial antibiotics.
CAI Guolin
,
FENG Wenxu
,
LIU Yifan
,
LI Xiaomin
,
LU Jian
. A potential probiotic Bacillus amyloliquefaciens strain producing exopolysaccharide with anti-hemagglutination activity towards enterotoxigenic Escherichia coli[J]. Food and Fermentation Industries, 2019
, 45(5)
: 14
-18
.
DOI: 10.13995/j.cnki.11-1802/ts.018363
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