The antifungal activities of limonoids against Penicillium were investigated by determining their minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC), and inhibition rates of spore germination and mycelium growth. By determining the changes of total sugar content, protein content, and alkaline phosphatase (AKP) activity, together with scanning electron microscope (SEM) observation, the antifungal mechanisms of limonoids were investigated. The results showed that limonoids had strong inhibitory activities against Penicillium. The MIC and MFC of limonoids were 625 μg/mL and 2 500 μg/mL, respectively. Their EC50 values of inhibiting mycelium growth and spore germination of Penicillium were 85.618 μg/mL and 246.755 μg/mL, respectively. Limonoids could destroy the cell wall and cell membrane of Penicillium in a short time, which caused a large amount of exudation of intracellular substances. Additionally, the higher concentration of limonoids, the more exudates of Penicillium. Moreover, SEM observation also indicated that Penicillium treated with limonoids had adherent exudates. Furthermore, the number of spores of the treated Penicillium decreased, and the spores also deformed. Besides, the pedicels damaged and cracked, and the mycelia became finer and deformed.
WANG Hui
,
ZENG Xiaofang
,
FENG Weihua
,
YU Limei
,
ZHAI Wanjing
,
BAI Weidong
,
ZENG Linggang
. Antifungal activity and mechanism of limonoids from lemon peel against Penicillium[J]. Food and Fermentation Industries, 2019
, 45(5)
: 75
-79
.
DOI: 10.13995/j.cnki.11-1802/ts.018422
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