Inulin in Jerusalem artichoke can improve intestinal function and has been widely used. Microorganisms have significant advantages in removing impurity components from inulin crude extracts. In this study, strain S-2 with low endoinulinase activity was screened from eleven yeast strains by measureing their inulase activity, and activities of endo-inulase and exo-inulase were determined by fructose standard curve using inulin and sucrose as substrates. Parameters that affect the removals of reducing sugar and protein from Jerusalem artichoke tuber extracts were optimized by orthogonal experiments. The results demonstrated that the removal rates of reducing sugar and protein by yeast strain S-2 were 92.4% and 92.9%, respectively, under the optimized conditions: cultivated at 30 ℃ for 50 h with 160 r/min rotational speed. Besides, the loss rate of inulin was 2.86% under this condition. The S-2 strain was identified as Saccharomyces cerevisiae by phylogenetic tree analysis. In conclusion, the removal effects of Saccharomyces cerevisiae S-2 on reducing sugar and protein were good, which could provide a theoretical basis for further research on impurity removal in industrial production of Jerusalem artichoke.
Li Xueyan
,
Wu Xiaoyao
,
Sun Chunli
,
Xie Huican
,
Wang Jinlong
. Microbial removal of impurities from Jerusalem artichoke and inulin crude extracts[J]. Food and Fermentation Industries, 2019
, 45(5)
: 127
-132
.
DOI: 10.13995/j.cnki.11-1802/ts.014557
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