Food and Fermentation Industries >

2019 , Vol. 45 >Issue 5: 218 - 223

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.017926

A fluorescent biosensor based on magnetic nanoparticles and aptamer for detecting AFM1 in milk

  • GUO Ting ,
  • LIN Shufeng ,
  • MA Liang ,
  • TAN Hongxia ,
  • ZHANG Yuhao
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  • (College of Food Science, Southwest University, Chongqing, 400715, China)

Online published: 2019-03-25

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Abstract

Based on the mechanism of fluorescence resonance energy transfer (FRET), a fluorescence biosensor was constructed by magnetic nanoparticles with abilities of magnetic separation and fluorescence quenching as well as the aptamer to highly sensitively detect aflatoxin M1 (AFM1) in milk. The aptamer labelled with carboxy-fluorescein (FAM) adsorbed to the surface of Fe3O4 magnetic nanoparticles by electrostatic interaction, followed by FRET from FAM-aptamer to Fe3O4, resulting in fluorescent quenching. When AFM1 was present, FAM-aptamer identified AFM1 specifically and desorbed from the surface of Fe3O4 after forming the aptamer-AFM1 complex, resulting the recovery of the fluorescent signal. Based on this, AFM1 could be quantitatively measured. In this study, the prepared Fe3O4 magnetic nanoparticles were characterized. Transmission electron microscopy analysis showed that the size of Fe3O4 was 10-15 nm. Under optimal conditions, the linear range of detection of the aptamer was 0.05-0.70 μg/L, and the detection limit was 0.02 μg/L. The recovery rate of using fluorescence biosensor to detect AFM1 in milk was 82.5%-102.3%. This developed strategy provides a new thought for constructing highly sensitive and rapid detection method.

Key words： aptamer; aflatoxin M1; Fe3O4 magnetic nanoparticles; fluorescence sensor

Cite this article

GUO Ting , LIN Shufeng , MA Liang , TAN Hongxia , ZHANG Yuhao . A fluorescent biosensor based on magnetic nanoparticles and aptamer for detecting AFM1 in milk[J]. Food and Fermentation Industries, 2019 , 45(5) : 218 -223 . DOI: 10.13995/j.cnki.11-1802/ts.017926

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