Partial characteristics of nattokinase produced by Bacillus subtilis MX-6

  • MAN Lili ,
  • XIANG Dianjun
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  • 1(College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028042, China)
    2 (College of Agriculture, Inner Mongolia University for Nationalities, Tongliao 028042, China)

Received date: 2018-10-30

  Online published: 2019-04-18

Abstract

The purpose of this study was to isolate and identify a strain with high production of streptokinase, and to analyze some characteristics of the streptokinase produced, including type, relative molecular mass, and fermentation curve. A high plasmin-production strain MX-6 was separated from Chinese douchi, which was identified as Bacillus subtilis by individual morphology, colony morphology, and homology comparison of 16S rDNA. The gene encoding plasmin in B. subtilis MX-6 (1 473 bp) was cloned by polymerase chain reaction (PCR). According to the results of multiple sequence alignment and phylogenetic tree between the deduced amino acid sequence and homologous sequences, the plasmin was identified as a nattokinase. The relative molecular mass of nattokinase produced by B. subtilis MX-6 was approximately 28 ku by SDS-polyacrylamide gels electrophoresis (SDS-PAGE), which coincided with the predicted molecular weight of 27 712.72 u. The fermentation curve of the nattokinase produced demonstrated that its maximum synthetic amount was obtained at 72 h, and the diameter of clear zone on the plasminogen-free fibrin plate was 21.60 mm. The results provide some theoretical basis and method reference for the development and application of nattokinase.

Cite this article

MAN Lili , XIANG Dianjun . Partial characteristics of nattokinase produced by Bacillus subtilis MX-6[J]. Food and Fermentation Industries, 2019 , 45(6) : 42 -47 . DOI: 10.13995/j.cnki.11-1802/ts.019194

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