This study aimed to evaluate the effects of pine pollen extracts on glycolipid metabolism in insulin-resistant HepG2 cells, and explore its underlying molecular mechanisms. The insulin-resistant HepG2 cell model was established by free fatty acids and validated using Oil red O staining. MTT assay was used to study the effects of pine pollen extracts with different polarities on cell proliferation. Levels of glucose, glycogen, and triglycerides (TG) in cell supernatants were measured. TG and total cholesterol (TC) in cytoplasm were also determined. Quantitative real-time PCR (qRT-PCR) was used to detect the mRNA expression levels of glucose and lipid metabolism-related genes in cell lysis buffer, such as InSR, PI3K and GLUT-4. The results showed that there were no obvious toxic effects of pine pollen extracts on HepG2 cells at 100-500 μg/mL. The absorption of glucose and transhipment of TG were promoted, and the degradation of glycogen and formations of TG and TC were suppressed after pine pollen extracts treatment, indicating that the insulin-resistance of HepG2 was improved. Meanwhile, the mRNA expressions of PI3K and GLUT-4 significantly increased in all pine pollen extracts treatment groups, which means that pine pollen could promote glucose metabolism by activating the PI3K/Akt/GLUT-4 pathway. It was also found that pine pollen after ethyl acetate, butanol and water treatments could attenuate insulin resistance through enhancing insulin sensitivity, because the mRNA expression of InSR up-regulated significantly among these three groups.
ZHAO Kexin
,
XIA Kai
,
YUAN Peng
,
WEN Lin
,
LI Aimin
,
LI Yongqiang
,
DUAN Shenglin
. Effects of pine pollen extracts on insulin resisted glycolipid metabolism in HepG2 cells[J]. Food and Fermentation Industries, 2019
, 45(6)
: 83
-90
.
DOI: 10.13995/j.cnki.11-1802/ts.018251
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