In order to clarify the inhibition mechanisms of pepper seed antifungal peptide (AMP) against corn- mildewing Aspergillus flavus, its minimum inhibitory concentration (MIC) and its effects on growth, spore germination, nutrient uptake, and cell membrane permeability of A. flavus were studied. The results showed that pepper seed AMP could inhibit the growth of A. flavus with the MIC of 156.25 μg/mL. At one-fold MIC, the germination rates of spores decreased to 18.51% and were completely inhibited at four-fold MIC. When AMP was added during the vegetative growth (in 24 h) of A. flavus, it could effectively inhibit spore formation. When AMP was added at different growth stages (0, 24, 48 h), it significantly reduced the ability of A. flavus to absorb nutrients from the medium. Sytox Green (SG) combined with a fluorescent microscope showed that the mycelium of AMP-treated A. flavus showed fluorescence, indicated that the cell membrane of A. flavus was damaged and therefore its permeability changed.
HAN Yuzhu
,
ZENG Bing
,
MENG Xing
,
WU Changfeng
,
LIAO Huanke
,
YANG Yemei
. Inhibitory effects of pepper seed antifungal peptide against Aspergillus flavus[J]. Food and Fermentation Industries, 2019
, 45(6)
: 110
-114
.
DOI: 10.13995/j.cnki.11-1802/ts.019137
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