Antibacterial activity and toxicity to human embryo kidney cells of silk fibroin peptides prepared by two kinds of enzymatic hydrolyses

  • CHENG Xifei ,
  • YANG Sha ,
  • CHEN Guo ,
  • ZHAO Yiping ,
  • PENG Yalin ,
  • ZHOU You ,
  • XU Li
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  • 1(College of Biotechnology, Southwest University, Chongqing 400715, China)
    2 (Chongqing Institute for Food and Drug Control, Chongqing 401121, China)

Received date: 2018-10-30

  Online published: 2019-04-18

Abstract

Silk fibroin powder was obtained from waste silkworm cocoon by degumming with Na2CO3, dissolving, dialysing and freeze-drying. The silk fibroin was hydrolyzed by alcalase and papain in distilled water. The silk fibroin peptides were obtained by inactivating the enzyme at 85 ℃. Reverse phase high performance liquid chromatography (RP-HPLC) was used to analyze the components of the fibroin peptides. Analyze the antimicrobial activity of ten silk fibroin peptides and the cytotoxicity to human embryonic kidney (HEK293) cells. It was found that P1, A2, A4, A1 silk fibroin peptides inhibited Escherichia coli and P1, P2, P3 silk fibroin peptides inhibited Staphylococcus aureus. These six silk fibroin peptides had no toxicity to HEK293 cell proliferation, and A1, A2, A4, P2 silk fibroin peptides could promote the survival of HEK293 cells, laying a theoretical foundation for the development, production and application of silk fibroin peptide in the future.

Cite this article

CHENG Xifei , YANG Sha , CHEN Guo , ZHAO Yiping , PENG Yalin , ZHOU You , XU Li . Antibacterial activity and toxicity to human embryo kidney cells of silk fibroin peptides prepared by two kinds of enzymatic hydrolyses[J]. Food and Fermentation Industries, 2019 , 45(6) : 115 -121 . DOI: 10.13995/j.cnki.11-1802/ts.019205

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