Three-phase partitioning (TPP), which is a simple, efficient, and green bioseparation technique, was used to extract and separate polysaccharides from Spriulina platensis (PSP). The effect of mass fraction of (NH4)2SO4, amount of t-butanol, pH, temperature, and extraction time on the yield of PSP were studied. The structural feature of extracted PSP were also analyzed. The optimal parameters with a high yield of (9.25±0.42)% were as follows: mass fraction of (NH4)2SO4, 20.0%; amount of t-butanol, 15 mL; pH 7.0; temperature, 35 ℃; and extraction time, 30 min. The results of IR showed that PSP had the characteristic absorption peaks of polysaccharide, UV wavelength scanning result showed that PSP do not contain protein and nucleic acids. The weight average molecular of PSP determined by gel permeation chromatography can reach 3.597×104, which is composed of Rhamnose, mannose, and glucose in a molar ratio of 5.14:5.78:81.37, respectively. Hence, TPP was an excellent efficient to concurrent extraction and purification polysaccharides.
LUO Guanghong
,
MA Minghui
,
ZHANG Xifeng
,
YANG Shenghui
,
WANG Danxia
,
CHEN Tianren
. Three-phase partitioning for efficient extraction and separation of polysaccharides from Spriulina platensis and its structural characterization[J]. Food and Fermentation Industries, 2019
, 45(6)
: 147
-152
.
DOI: 10.13995/j.cnki.11-1802/ts.017786
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