D-phenyllactic acid is an ideal natural antimicrobial compound with broad spectrum activity. A lactate dehydrogenase recombinant bacteria Escherichia coli BL21(DE3)/pET28a-ldh was constructed, and its activity under optimized conditions reached 298.8 U/g dry cell weight. A lactate dehydrogenase and glucose dehydrogenase coupled catalysis system was established and applied in reducing sodium pyruvate to produce D-phenyllactic acid. The reaction conditions were optimized, including temperature, pH, mass ratio of two recombinant bacteria, and glucose concentration. Under the optimal reaction condition, D-phenyllactic acid accumulated up to 18.03 g/L with ee >99%, and the space-time yield reached 162.27 g/(L·d). Therefore, this catalytic system is effective to synthesize D-phenyllactic acid and has shown a good application prospect.
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